This paper was published in Biomedical Optics Express and is made available as an electronic reprint with the permission of OSA. The paper can be found at the following URL on the OSA website: http://www.opticsinfobase.org/boe/abstract.cfm?uri=boe-2-1-113. Systematic or multiple reproduction or distribution to multiple locations via electronic or other means is prohibited and is subject to penalties under law. See the OSA Author Copyright Permissions page for further information.
We present the application of remote focusing to multiphoton laser scanning microscopy and utilize this technology to demonstrate simultaneous, programmable multi-layer imaging. Remote focusing is used to independently control the axial location of multiple focal planes that can be simultaneously imaged with single element detection. This facilitates volumetric multiphoton imaging in scattering specimens and can be practically scaled to a large number of focal planes. Further, it is demonstrated that the remote focusing control can be synchronized with the lateral scan directions, enabling imaging in orthogonal scan planes.
Erich E. Hoover, Michael D. Young, Eric V. Chandler, Anding Luo, Jeffrey J. Field, Kraig E. Sheetz, Anne W. Sylvester, and Jeff A. Squier, "Remote focusing for programmable multi-layer differential multiphoton microscopy," Biomed. Opt. Express 2, 113-122 (2011)
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